David C Bloom

David C Bloom

PROFESSOR

Department: Molecular Genetics & Microbiology
Business Phone: (352) 273-9524
Business Email: dbloom@ufl.edu

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0001-5935-8494

Publications

2020
Deletion of Herpes Simplex Virus 1 MicroRNAs miR-H1 and miR-H6 Impairs Reactivation.
Journal of virology. 94(15) [DOI] 10.1128/JVI.00639-20. [PMID] 32295910.
2020
Patterns of Herpes Simplex Virus 1 Infection in Neural Progenitor Cells.
Journal of virology. 94(16) [DOI] 10.1128/JVI.00994-20. [PMID] 32493817.
2020
Modeling Aβ42 Accumulation in Response to Herpes Simplex Virus 1 Infection: 2D or 3D?
Journal of virology. [DOI] 10.1128/JVI.02219-20. [PMID] 33268524.
2020
Herpes Simplex Virus 1 microRNA miR-H8 is Dispensable for Latency and Reactivation in Vivo.
Journal of virology. [DOI] 10.1128/JVI.02179-20. [PMID] 33208453.
2020
Herpes Simplex Virus 1 Strains 17syn+ and KOS(M) Differ Greatly in Their Ability To Reactivate from Human Neurons In Vitro.
Journal of virology. 94(15) [DOI] 10.1128/JVI.00796-20. [PMID] 32461310.
2020
Herpes Simplex Viruses Whose Replication Can Be Deliberately Controlled as Candidate Vaccines.
Vaccines. 8(2) [DOI] 10.3390/vaccines8020230. [PMID] 32443425.
2019
Lund Human Mesencephalic (LUHMES) Neuronal Cell Line Supports Herpes Simplex Virus 1 Latency In Vitro.
Journal of virology. 93(6) [DOI] 10.1128/JVI.02210-18. [PMID] 30602607.
2019
Modeling Herpes Simplex Virus 1 Infections in Human Central Nervous System Neuronal Cells Using Two- and Three-Dimensional Cultures Derived from Induced Pluripotent Stem Cells.
Journal of virology. 93(9) [DOI] 10.1128/JVI.00111-19. [PMID] 30787148.
2019
Perillyl alcohol and perillic acid exert efficient action upon HSV-1 maturation and release of infective virus
Antiviral Therapy. 25(1):1-11 [DOI] 10.3851/IMP3315. [PMID] 31099756.
2019
Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia.
Methods in molecular biology (Clifton, N.J.). 1950:237-247 [DOI] 10.1007/978-1-4939-9139-6_13. [PMID] 30783977.
2019
Personalized viral genomic investigation of herpes simplex virus 1 perinatal viremic transmission with dual fatality.
Cold Spring Harbor molecular case studies. 5(6) [DOI] 10.1101/mcs.a004382. [PMID] 31582464.
2019
The CCCTC Binding Factor, CTRL2, Modulates Heterochromatin Deposition and the Establishment of Herpes Simplex Virus 1 Latency In Vivo.
Journal of virology. 93(13) [DOI] 10.1128/JVI.00415-19. [PMID] 30996085.
2019
Virtual screening identified compounds that bind to cyclin dependent kinase 2 and prevent herpes simplex virus type 1 replication and reactivation in neurons.
Antiviral research. 172 [DOI] 10.1016/j.antiviral.2019.104621. [PMID] 31634495.
2018
Reactivation of Latent Epstein-Barr Virus: A Comparison after Exposure to Gamma, Proton, Carbon, and Iron Radiation.
International journal of molecular sciences. 19(10) [DOI] 10.3390/ijms19102961. [PMID] 30274169.
2018
In Vivo Knockdown of the Herpes Simplex Virus 1 Latency-Associated Transcript Reduces Reactivation from Latency.
Journal of virology. 92(16) [DOI] 10.1128/JVI.00812-18. [PMID] 29875240.
2018
Immunization by Replication-Competent Controlled Herpesvirus Vectors.
Journal of virology. 92(16) [DOI] 10.1128/JVI.00616-18. [PMID] 29899091.
2018
The ATM and Rad3-Related (ATR) Protein Kinase Pathway Is Activated by Herpes Simplex Virus 1 and Required for Efficient Viral Replication.
Journal of virology. 92(6) [DOI] 10.1128/JVI.01884-17. [PMID] 29263259.
2018
R430: A potent inhibitor of DNA and RNA viruses.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-33904-y. [PMID] 30413769.
2018
Generation of Three-Dimensional Human Neuronal Cultures: Application to Modeling CNS Viral Infections
Current Stem Cell Research & Therapy. 9(1) [DOI] 10.1186/s13287-018-0881-6. [PMID] 29751846.
2018
Depletion of the Insulator Protein CTCF Results in Herpes Simplex Virus 1 Reactivation In Vivo.
Journal of virology. 92(11) [DOI] 10.1128/JVI.00173-18. [PMID] 29514910.
2018
Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 From Multiple Anatomic Sites Over Time.
The Journal of infectious diseases. 218(4):595-605 [DOI] 10.1093/infdis/jiy216. [PMID] 29920588.
2017
Comparison of three cell-based drug screening platforms for HSV-1 infection.
Antiviral research. 142:136-140 [DOI] 10.1016/j.antiviral.2017.03.016. [PMID] 28342892.
2017
Development of Recombinant HSV-Based Vaccine Vectors.
Methods in molecular biology (Clifton, N.J.). 1581:55-78 [DOI] 10.1007/978-1-4939-6869-5_4. [PMID] 28374243.
2017
HSV1 latent transcription and non-coding RNA: A critical retrospective.
Journal of neuroimmunology. 308:65-101 [DOI] 10.1016/j.jneuroim.2017.03.002. [PMID] 28363461.
2017
Reliable Detection of Herpes Simplex Virus Sequence Variation by High-Throughput Resequencing.
Viruses. 9(8) [DOI] 10.3390/v9080226. [PMID] 28812996.
2016
Simultaneous visualization of two Citrus tristeza virus genotypes provides new insights into the structure of multi-component virus populations in a host.
Virology. 491:10-9 [DOI] 10.1016/j.virol.2016.01.017. [PMID] 26874013.
2016
Herpes simplex virus type-1: replication, latency, reactivation and its antiviral targets.
Antiviral therapy. 21(4):277-86 [DOI] 10.3851/IMP3018. [PMID] 26726828.
2016
Adeno-associated Virus Vectors Efficiently Transduce Mouse and Rabbit Sensory Neurons Coinfected with Herpes Simplex Virus 1 following Peripheral Inoculation.
Journal of virology. 90(17):7894-901 [DOI] 10.1128/JVI.01028-16. [PMID] 27334582.
2016
Alphaherpesvirus Latency: A Dynamic State of Transcription and Reactivation.
Advances in virus research. 94:53-80 [DOI] 10.1016/bs.aivir.2015.10.001. [PMID] 26997590.
2015
A Genomic Approach to Unravel Host-Pathogen Interaction in Chelonians: The Example of Testudinid Herpesvirus 3.
PloS one. 10(8) [DOI] 10.1371/journal.pone.0134897. [PMID] 26244892.
2015
A novel approach for addressing diseases not yielding to effective vaccination? Immunization by replication-competent controlled virus.
Expert review of vaccines. 14(5):637-51 [DOI] 10.1586/14760584.2015.1013941. [PMID] 25676927.
2015
Broad-Spectrum Non-Nucleoside Inhibitors of Human Herpesviruses
Antiviral Research. 121:16-23 [DOI] 10.1016/j.antiviral.2015.06.005. [PMID] 26079681.
2015
Inhibition of H3K27me3-specific histone demethylases JMJD3 and UTX blocks reactivation of herpes simplex virus 1 in trigeminal ganglion neurons.
Journal of virology. 89(6):3417-20 [DOI] 10.1128/JVI.03052-14. [PMID] 25552720.
2015
Replication-Competent Controlled Herpes Simplex Virus.
Journal of virology. 89(20):10668-79 [DOI] 10.1128/JVI.01667-15. [PMID] 26269179.
2014
Leveraging biological replicates to improve analysis in ChIP-seq experiments.
Computational and structural biotechnology journal. 9 [DOI] 10.5936/csbj.201401002. [PMID] 24688750.
2013
Mutational inactivation of herpes simplex virus 1 microRNAs identifies viral mRNA targets and reveals phenotypic effects in culture.
Journal of virology. 87(12):6589-603 [DOI] 10.1128/JVI.00504-13. [PMID] 23536669.
2013
Role of polycomb proteins in regulating HSV-1 latency.
Viruses. 5(7):1740-57 [DOI] 10.3390/v5071740. [PMID] 23860385.
2012
Regulated protein expression for in vivo gene therapy for neurological disorders: progress, strategies, and issues.
Neurobiology of disease. 48(2):212-21 [DOI] 10.1016/j.nbd.2012.03.001. [PMID] 22426391.
2012
Respiratory function following bilateral mid-cervical contusion injury in the adult rat.
Experimental neurology. 235(1):197-210 [DOI] 10.1016/j.expneurol.2011.09.024. [PMID] 21963673.
2012
LAT region factors mediating differential neuronal tropism of HSV-1 and HSV-2 do not act in trans.
PloS one. 7(12) [DOI] 10.1371/journal.pone.0053281. [PMID] 23300908.
2011
Three Group Risk Stratification for Pediatric Brain Stem Gliomas Treated With Radiotherapy Based On Clinical Presentation and Imaging Characteristics
Pediatric Blood & Cancer. 57:720-721
2010
Braun Enteroenterostomy Is Associated With Reduced Delayed Gastric Emptying and Early Resumption of Oral Feeding Following Pancreaticoduodenectomy
Journal of Surgical Oncology. 101(5):351-355 [DOI] 10.1002/jso.21490. [PMID] 20112274.
2010
Epigenetic regulation of latent HSV-1 gene expression.
Biochimica et biophysica acta. 1799(3-4):246-56 [DOI] 10.1016/j.bbagrm.2009.12.001. [PMID] 20045093.
2009
A limited innate immune response is induced by a replication-defective herpes simplex virus vector following delivery to the murine central nervous system.
Journal of neurovirology. 15(5-6):411-24 [DOI] 10.3109/13550280903473452. [PMID] 20095947.
2009
Fragile X mental retardation protein replacement restores hippocampal synaptic function in a mouse model of fragile X syndrome.
Gene therapy. 16(9):1122-9 [DOI] 10.1038/gt.2009.83. [PMID] 19571888.
2009
The polycomb group protein Bmi1 binds to the herpes simplex virus 1 latent genome and maintains repressive histone marks during latency.
Journal of virology. 83(16):8173-81 [DOI] 10.1128/JVI.00686-09. [PMID] 19515780.
2008
Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy.
Molecular vision. 14:2087-96 [PMID] 19023450.
View on: PubMed
2008
During herpes simplex virus type 1 infection of rabbits, the ability to express the latency-associated transcript increases latent-phase transcription of lytic genes.
Journal of virology. 82(12):6056-60 [DOI] 10.1128/JVI.02661-07. [PMID] 18400860.
2008
Fak and Igf-Ir Interact To Provide Survival Signals in Human Pancreatic Adenocarcinoma Cells
Carcinogenesis. 29(6):1096-1107 [DOI] 10.1093/carcin/bgn026. [PMID] 18263593.
2008
Microarray analysis of host gene expression for comparison between naïve and HSV-1 latent rabbit trigeminal ganglia.
Molecular vision. 14:1209-21 [PMID] 18615202.
View on: PubMed
2008
Ranavirus infection of free-ranging and captive box turtles and tortoises in the United States.
Journal of wildlife diseases. 44(4):851-63 [PMID] 18957641.
View on: PubMed
2008
Reduction in severity of a herpes simplex virus type 1 murine infection by treatment with a ribozyme targeting the UL20 gene RNA.
Journal of virology. 82(15):7467-74 [DOI] 10.1128/JVI.02720-07. [PMID] 18508896.
2008
Cervical prephrenic interneurons in the normal and lesioned spinal cord of the adult rat.
The Journal of comparative neurology. 511(5):692-709 [DOI] 10.1002/cne.21864. [PMID] 18924146.
2007
In vivo changes in the patterns of chromatin structure associated with the latent herpes simplex virus type 1 genome in mouse trigeminal ganglia can be detected at early times after butyrate treatment.
Journal of virology. 81(23):13248-53 [PMID] 17881451.
View on: PubMed
2006
Tissue-specific splicing of the herpes simplex virus type 1 latency-associated transcript (LAT) intron in LAT transgenic mice.
Journal of virology. 80(19):9414-23 [PMID] 16973547.
View on: PubMed
2006
Deacetylation of the herpes simplex virus type 1 latency-associated transcript (LAT) enhancer and a decrease in LAT abundance precede an increase in ICP0 transcriptional permissiveness at early times postexplant.
Journal of virology. 80(4):2063-8 [PMID] 16439563.
View on: PubMed
2006
A chromatin insulator-like element in the herpes simplex virus type 1 latency-associated transcript region binds CCCTC-binding factor and displays enhancer-blocking and silencing activities.
Journal of virology. 80(5):2358-68 [PMID] 16474142.
View on: PubMed
2005
Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods.
Folia morphologica. 64(3):130-44 [PMID] 16228947.
View on: PubMed
2005
Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine.
Brain research. Molecular brain research. 139(2):361-6 [PMID] 16039006.
View on: PubMed
2004
Experimental transmission of a herpesvirus in Greek tortoises (Testudo graeca).
Veterinary pathology. 41(1):50-61 [PMID] 14715968.
View on: PubMed
2004
HSV LAT and neuronal survival.
International reviews of immunology. 23(1-2):187-98 [PMID] 14690860.
View on: PubMed
2004
Specific histone tail modification and not DNA methylation is a determinant of herpes simplex virus type 1 latent gene expression.
Journal of virology. 78(3):1139-49 [PMID] 14722269.
View on: PubMed
2004
The herpes simplex virus type 1 latency-associated transcript (LAT) enhancer/rcr is hyperacetylated during latency independently of LAT transcription.
Journal of virology. 78(22):12508-18 [PMID] 15507638.
View on: PubMed
2004
Wide variations in herpes simplex virus type 1 inoculum dose and latency-associated transcript expression phenotype do not alter the establishment of latency in the rabbit eye model.
Journal of virology. 78(10):5038-44 [PMID] 15113885.
View on: PubMed
2003
Herpes simplex virus-1 and varicella-zoster virus latency in ganglia.
Journal of neurovirology. 9(2):194-204 [PMID] 12707850.
View on: PubMed
2003
Overlapping subdeletions within a 348-bp in the 5′ exon of the LAT region that facilitates epinephrine-induced reactivation of HSV-1 in the rabbit ocular model do not further define a functional element.
Virology. 312(1):151-8 [PMID] 12890628.
View on: PubMed
2002
Altering the expression kinetics of VP5 results in altered virulence and pathogenesis of herpes simplex virus type 1 in mice.
Journal of virology. 76(5):2199-205 [PMID] 11836397.
View on: PubMed
2002
Site-directed mutagenesis of cysteine to serine in the DNA binding region of Nrf2 decreases its capacity to upregulate antioxidant response element-mediated expression and antioxidant induction of NAD(P)H:quinone oxidoreductase1 gene.
Oncogene. 21(14):2191-200 [PMID] 11948402.
View on: PubMed
2002
The region of the HSV-1 latency-associated transcript required for epinephrine-induced reactivation in the rabbit does not include the 2.0-kb intron.
Virology. 292(1):59-69 [PMID] 11878908.
View on: PubMed
1993
A revised HindIII map and sequence analysis of a large ‘left-hand’ non-essential region of the rabbit poxvirus genome.
Virus research. 28(2):125-40 [PMID] 8391191.
View on: PubMed
1991
Identification and characterization of two nonessential regions of the rabbitpox virus genome involved in virulence.
Journal of virology. 65(3):1530-42 [PMID] 1847470.
View on: PubMed
1991
The nature of naturally occurring mutations in the hemagglutinin gene of vaccinia virus and the sequence of immediately adjacent genes.
Virus genes. 5(3):235-42 [PMID] 1771764.
View on: PubMed

Grants

Dec 2020 ACTIVE
Basic Microbiology and Infectious Disease
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Sep 2020 – Dec 2020
Assessment of Reactivation of HSV Oncolytic Vectors
Role: Principal Investigator
Funding: VIROGIN BIOTECH CANADA
Jul 2020 – Aug 2020
Analysis of Hypochlorous Acid Wound Cleanser for Anti-Viral Activity
Role: Principal Investigator
Funding: URGO MEDICAL
Jul 2020 ACTIVE
Effects of HSV-1 infection on neural progenitor cell biology in vitro and in vivo
Role: Principal Investigator
Funding: UNIV OF PITTSBURGH via NATL INST OF HLTH NINDS
Apr 2020 – Jun 2020
Analysis of Nanocrystalline Silver for Anti-viral Activity
Role: Principal Investigator
Funding: UNIV OF ALBERTA
Jan 2020 ACTIVE
RNA polymerase II occupancy and activity in HSV-infected post-mitotic neurons
Role: Principal Investigator
Funding: LOUISIANA STATE UNIV via NATL INST OF HLTH NIAID
Aug 2019 ACTIVE
Phenotypic analyses of HSV-1 miRNAs: H1, H6, & H8
Role: Other
Funding: NATL INST OF HLTH NIAID
Mar 2019 ACTIVE
Molecular Genetics of HSV Reactivation
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Nov 2018 ACTIVE
CTCF-mediated HSV-1 gene expression during latency and reactivation
Role: Principal Investigator
Funding: UNIV OF WISCONSIN MADISON via NATL INST OF HLTH NIAID
Oct 2018 – May 2020
Analysis of the specific effects of Tranexamic Acid on HSV infection
Role: Principal Investigator
Funding: ANTI PLASMIN TECHNOLOGIES
Sep 2016 – Aug 2018
Analysis of human-specific aspects of HSV-1 latency in human iPSC-derived neurons
Role: Principal Investigator
Funding: UNIV OF PITTSBURGH via NATL INST OF HLTH NINDS
Jun 2016 – May 2018
OR-DRPD-ROF2016: Visualizing viral entry into neurons and the brain: Changing the paradigm
Role: Co-Investigator
Funding: UF DSR OPPORTUNITY FUND
Jan 2016 – Dec 2016
Vector-delivered CRISPR/Cas as a cure for HSV-1 induced keratitis
Role: Principal Investigator
Funding: EDITAS MEDICINE via NATL INST OF HLTH NIAID
Sep 2015 – Aug 2016
The Role of the LAT Locus in HSV-1 Infection of Human Skin Xenografts in Vivo
Role: Principal Investigator
Funding: STANFORD UNIV via NATL INST OF HLTH NIAID
Jul 2015 – Jun 2018
Function of histone chaperones in HSV-1 chromatin sturcture during latency, establishing maintenance and reactivation
Role: Project Manager
Funding: NATL INST OF HLTH NIAID
Jun 2015 – Jun 2016
Effect of spaceflight on Herpesvirus genome stability and diversity
Role: Principal Investigator
Funding: NASA
Aug 2014 – Jul 2020
Basic Microbiology and Infectious Disease
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Aug 2014 – Apr 2017
Cytotoxic lymphocytes and HSV-1 corneal lesions
Role: Principal Investigator
Funding: UNIV OF PITTSBURGH via NATL INST OF HLTH
Jan 2014 – Jan 2017
Determination of Roles of Microgravity and Ionizing Radiation on the Reactivation of Epstein-Barr Virus in vitro
Role: Principal Investigator
Funding: NASA STENNIS SPACE FLIGHT CTR
Jul 2013 ACTIVE
Evaluation of the suitability of SafeSwitch-controlled HSV as anti-herpetic"vaccines" or as immunization platform
Role: Principal Investigator
Funding: HSF PHARMACEUTICALS SA
Jan 2012 – Dec 2017
Regulation of lytic and latent infection by HSV-1 encoded miRNAs
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Jul 2010 – Oct 2020
HSV-1 Research Program – UFF Transfer Account
Role: Principal Investigator
Funding: UF FOU
Jul 2010 – Oct 2020
HSV-2 Research Program – UFF Transfer Account
Role: Principal Investigator
Funding: UF FOU

Patents

Issued May 1993
Biological System for Constructing and Testing Viral Vaccines Culture Of Poxvirus and Plasmids
#5,212,057
Issued February 2008
Herpesvirus Ribozymes and Vectors
#7,335,763
Issued November 2010
Herpesvirus Ribozymes and Vectors (CIP)
#7,838,659
Issued May 2015
Insulated Herpesvirus-derived Gene Expression Cassette for Sustained and Regulatable Gene Expression
#9,023,617
Published September 2016
Method for the Treatment of Latent Infection with HSV-1 or HSV-2
#US2017/0020994
Issued May 2017
Insulated Herpesvirus-derived Gene Expression Cassette for Sustained and Regulatable Gene Expression (DIV 11434)
#9,636,354

Teaching Profile

Courses Taught
2020-2021
GMS7133 Advanced Molecular Virology
2020-2021
BMS6634 GI and Hepatology
2008,2010-2021
GMS6196 Virology Journal Club
2013-2021
GMS6035 Advanced Virology II: RNA Viruses
2012-2019
GMS5905 Special Topics in Biomedical Sciences
2019
EGN4912 Engineering Directed Independent Research
2013,2015-2018
BCH7412 Epigenetics of Human Disease and Development
2018
GMS7980 Research for Doctoral Dissertation
2018
GMS6034 Advanced Virology I: Genetics and RNA
2018
BMS6300 Fund Micro and Immuno
2006-2007
GMS6181 Spec Topics Microbiol

Contact Details

Phones:
Business:
(352) 273-9524
Emails:
Business:
dbloom@ufl.edu