L J Brady

L J Brady,

Professor & Director Of Graduate And Post-Doctoral Research Training And Career Development

Department: DN-ORAL BIOLOGY
Business Phone: (352) 273-8839
Business Email: jbrady@dental.ufl.edu

Publications

2021
Amyloid Aggregation of Streptococcus mutans Cnm Influences Its Collagen-Binding Activity.
Applied and environmental microbiology. 87(21) [DOI] 10.1128/AEM.01149-21. [PMID] 34406827.
2021
Protein Interactomes of Streptococcus mutans YidC1 and YidC2 Membrane Protein Insertases Suggest SRP Pathway-Independent- and -Dependent Functions, Respectively.
mSphere. 6(2) [DOI] 10.1128/mSphere.01308-20. [PMID] 33658280.
2021
The Cytoplasmic Domains of Streptococcus mutans Membrane Protein Insertases YidC1 and YidC2 Confer Unique Structural and Functional Attributes to Each Paralog.
Frontiers in microbiology. 12 [DOI] 10.3389/fmicb.2021.760873. [PMID] 34795653.
2020
Characterization of an intermolecular quaternary interaction between discrete segments of the Streptococcus mutans adhesin P1 by NMR spectroscopy.
The FEBS journal. 287(12):2597-2611 [DOI] 10.1111/febs.15158. [PMID] 31782893.
2020
Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids.
Scientific reports. 10(1) [DOI] 10.1038/s41598-020-62115-7. [PMID] 32198417.
2020
Multiple Factors are Involved in Regulation of Extracellular Membrane Vesicle Biogenesis in Streptococcus mutans
Molecular oral microbiology. 36(1):12-24 [DOI] 10.1111/omi.12318. [PMID] 33040492.
2020
The Impacts of Sortase A and the 4′-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis.
Frontiers in microbiology. 11 [DOI] 10.3389/fmicb.2020.570219. [PMID] 33193163.
2019
Membrane proteomic analysis reveals overlapping and independent functions of Streptococcus mutans Ffh, YidC1, and YidC2.
Molecular oral microbiology. 34(4):131-152 [DOI] 10.1111/omi.12261. [PMID] 31034136.
2019
Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.
Journal of bacteriology. 201(1) [DOI] 10.1128/JB.00396-18. [PMID] 30322852.
2019
The Biology of Streptococcus mutans.
Microbiology spectrum. 7(1) [DOI] 10.1128/microbiolspec.GPP3-0051-2018. [PMID] 30657107.
2018
Biology of Oral Streptococci.
Microbiology spectrum. 6(5) [DOI] 10.1128/microbiolspec.GPP3-0042-2018. [PMID] 30338752.
2018
Characterization of the pgf operon involved in the posttranslational modification of Streptococcus mutans surface proteins.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-23170-3. [PMID] 29549320.
2017
Functional amyloids in Streptococcus mutans, their use as targets of biofilm inhibition and initial characterization of SMU_63c.
Microbiology (Reading, England). 163(4):488-501 [DOI] 10.1099/mic.0.000443. [PMID] 28141493.
2017
K+ modulates genetic competence and the stress regulon of Streptococcus mutans.
Microbiology (Reading, England). 163(5):719-730 [DOI] 10.1099/mic.0.000458. [PMID] 28530170.
2017
LT adjuvant modulates epitope specificity and improves the efficacy of murine antibodies elicited by sublingual vaccination with the N-terminal domain of Streptococcus mutans P1.
Vaccine. 35(52):7273-7282 [DOI] 10.1016/j.vaccine.2017.11.007. [PMID] 29146379.
2016
Evaluation of the effects of Streptococcus mutans chaperones and protein secretion machinery components on cell surface protein biogenesis, competence, and mutacin production.
Molecular oral microbiology. 31(1):59-77 [DOI] 10.1111/omi.12130. [PMID] 26386361.
2016
Specific binding of a naturally occurring amyloidogenic fragment of Streptococcus mutans adhesin P1 to intact P1 on the cell surface characterized by solid state NMR spectroscopy.
Journal of biomolecular NMR. 64(2):153-64 [DOI] 10.1007/s10858-016-0017-1. [PMID] 26837620.
2016
Trk2 Potassium Transport System in Streptococcus mutans and Its Role in Potassium Homeostasis, Biofilm Formation, and Stress Tolerance.
Journal of bacteriology. 198(7):1087-100 [DOI] 10.1128/JB.00813-15. [PMID] 26811321.
2015
Binding forces of Streptococcus mutans P1 adhesin.
ACS nano. 9(2):1448-60 [DOI] 10.1021/nn5058886. [PMID] 25671413.
2015
Breaking the bacterial protein targeting and translocation model: oral organisms as a case in point.
Molecular oral microbiology. 30(3):186-97 [DOI] 10.1111/omi.12088. [PMID] 25400073.
2015
Identification of a supramolecular functional architecture of Streptococcus mutans adhesin P1 on the bacterial cell surface.
The Journal of biological chemistry. 290(14):9002-19 [DOI] 10.1074/jbc.M114.626663. [PMID] 25666624.
2014
Alterations in immunodominance of Streptococcus mutans AgI/II: lessons learned from immunomodulatory antibodies.
Vaccine. 32(3):375-82 [DOI] 10.1016/j.vaccine.2013.11.023. [PMID] 24252705.
2014
An intramolecular lock facilitates folding and stabilizes the tertiary structure of Streptococcus mutans adhesin P1.
Proceedings of the National Academy of Sciences of the United States of America. 111(44):15746-51 [DOI] 10.1073/pnas.1413018111. [PMID] 25331888.
2014
Immunogenicity and in vitro and in vivo protective effects of antibodies targeting a recombinant form of the Streptococcus mutans P1 surface protein.
Infection and immunity. 82(12):4978-88 [DOI] 10.1128/IAI.02074-14. [PMID] 25225243.
2014
Streptococcus mutans extracellular DNA is upregulated during growth in biofilms, actively released via membrane vesicles, and influenced by components of the protein secretion machinery.
Journal of bacteriology. 196(13):2355-66 [DOI] 10.1128/JB.01493-14. [PMID] 24748612.
2014
YlxM is a newly identified accessory protein that influences the function of signal recognition particle pathway components in Streptococcus mutans.
Journal of bacteriology. 196(11):2043-52 [DOI] 10.1128/JB.01465-13. [PMID] 24659773.
2013
An intramolecular interaction involving the N terminus of a streptococcal adhesin affects its conformation and adhesive function.
The Journal of biological chemistry. 288(19):13762-74 [DOI] 10.1074/jbc.M113.459974. [PMID] 23539625.
2012
Brpa Is Involved in Regulation of Cell Envelope Stress Responses in Streptococcus Mutans
Applied and Environmental Microbiology. 78(8):2914-2922 [DOI] 10.1128/AEM.07823-11. [PMID] 22327589.
2012
Functional amyloid formation by Streptococcus mutans.
Microbiology (Reading, England). 158(Pt 12):2903-2916 [DOI] 10.1099/mic.0.060855-0. [PMID] 23082034.
2012
YidC1 and YidC2 are functionally distinct proteins involved in protein secretion, biofilm formation and cariogenicity of Streptococcus mutans.
Microbiology (Reading, England). 158(Pt 7):1702-1712 [DOI] 10.1099/mic.0.059139-0. [PMID] 22504439.
2011
A therapeutic anti-Streptococcus mutans monoclonal antibody used in human passive protection trials influences the adaptive immune response.
Vaccine. 29(37):6292-300 [DOI] 10.1016/j.vaccine.2011.06.027. [PMID] 21704107.
2011
Crystal structure of the C-terminal region of Streptococcus mutans antigen I/II and characterization of salivary agglutinin adherence domains.
The Journal of biological chemistry. 286(24):21657-66 [DOI] 10.1074/jbc.M111.231100. [PMID] 21505225.
2010
Elongated fibrillar structure of a streptococcal adhesin assembled by the high-affinity association of alpha- and PPII-helices.
Proceedings of the National Academy of Sciences of the United States of America. 107(13):5983-8 [DOI] 10.1073/pnas.0912293107. [PMID] 20231452.
2010
Induction of neutralizing antibodies in mice immunized with an amino-terminal polypeptide of Streptococcus mutans P1 protein produced by a recombinant Bacillus subtilis strain.
FEMS immunology and medical microbiology. 59(2):131-42 [DOI] 10.1111/j.1574-695X.2010.00669.x. [PMID] 20402772.
2010
The changing faces of Streptococcus antigen I/II polypeptide family adhesins.
Molecular microbiology. 77(2):276-86 [DOI] 10.1111/j.1365-2958.2010.07212.x. [PMID] 20497507.
2009
Beneficial immunomodulation by Streptococcus mutans anti-P1 monoclonal antibodies is Fc independent and correlates with increased exposure of a relevant target epitope.
Journal of immunology (Baltimore, Md. : 1950). 183(7):4628-38 [DOI] 10.4049/jimmunol.0803300. [PMID] 19752237.
2009
Independent gene duplications of the YidC/Oxa/Alb3 family enabled a specialized cotranslational function.
Proceedings of the National Academy of Sciences of the United States of America. 106(16):6656-61 [DOI] 10.1073/pnas.0809951106. [PMID] 19366667.
2008
Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva.
Infection and immunity. 76(9):4259-68 [DOI] 10.1128/IAI.00422-08. [PMID] 18625741.
2008
Functional overlap but lack of complete cross-complementation of Streptococcus mutans and Escherichia coli YidC orthologs.
Journal of bacteriology. 190(7):2458-69 [DOI] 10.1128/JB.01366-07. [PMID] 18178746.
2008
Isolation and solubilization of cellular membrane proteins from bacteria.
Methods in molecular biology (Clifton, N.J.). 425:287-93 [DOI] 10.1007/978-1-60327-210-0_23. [PMID] 18369904.
2008
Requirements for surface expression and function of adhesin P1 from Streptococcus mutans.
Infection and immunity. 76(6):2456-68 [DOI] 10.1128/IAI.01315-07. [PMID] 18362133.
2007
Basis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1.
FEMS immunology and medical microbiology. 51(1):102-11 [PMID] 17614961.
View on: PubMed
2007
Characterization of epitopes recognized by anti-Streptococcus mutans P1 monoclonal antibodies.
FEMS immunology and medical microbiology. 50(3):342-53 [PMID] 17535300.
View on: PubMed
2007
Consequences of a sortase A mutation in Streptococcus gordonii.
Microbiology (Reading, England). 153(Pt 12):4088-4097 [DOI] 10.1099/mic.0.2007/007252-0. [PMID] 18048922.
2007
Membrane composition changes and physiological adaptation by Streptococcus mutans signal recognition particle pathway mutants.
Journal of bacteriology. 189(4):1219-30 [PMID] 17085548.
View on: PubMed
2006
A unique serine-rich repeat protein (Srr-2) and novel surface antigen (epsilon) associated with a virulent lineage of serotype III Streptococcus agalactiae.
Microbiology (Reading, England). 152(Pt 4):1029-1040 [DOI] 10.1099/mic.0.28516-0. [PMID] 16549667.
2006
A whole cell BIAcore assay to evaluate P1-mediated adherence of Streptococcus mutans to human salivary agglutinin and inhibition by specific antibodies.
Journal of microbiological methods. 65(3):503-11 [PMID] 16239043.
View on: PubMed
2005
Antibody-mediated immunomodulation: a strategy to improve host responses against microbial antigens.
Infection and immunity. 73(2):671-8 [PMID] 15664904.
View on: PubMed
2005
Solubilization of cellular membrane proteins from Streptococcus mutans for two-dimensional gel electrophoresis.
Electrophoresis. 26(6):1200-5 [PMID] 15706571.
View on: PubMed
2005
Streptococcal viability and diminished stress tolerance in mutants lacking the signal recognition particle pathway or YidC2.
Proceedings of the National Academy of Sciences of the United States of America. 102(48):17466-71 [PMID] 16293689.
View on: PubMed
2004
An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture.
FEMS microbiology letters. 234(2):315-24 [PMID] 15135539.
View on: PubMed
2004
Characterization of the Streptococcus mutans P1 epitope recognized by immunomodulatory monoclonal antibody 6-11A.
Infection and immunity. 72(8):4680-8 [PMID] 15271929.
View on: PubMed
2004
Contribution of the alanine-rich region of Streptococcus mutans P1 to antigenicity, surface expression, and interaction with the proline-rich repeat domain.
Infection and immunity. 72(8):4699-706 [PMID] 15271931.
View on: PubMed
2004
Further characterization of immunomodulation by a monoclonal antibody against Streptococcus mutans antigen P1.
Infection and immunity. 72(1):13-21 [PMID] 14688075.
View on: PubMed
2004
Redirecting the humoral immune response against Streptococcus mutans antigen P1 with monoclonal antibodies.
Infection and immunity. 72(12):6951-60 [PMID] 15557617.
View on: PubMed
2003
Characterization of group B streptococcal glyceraldehyde-3-phosphate dehydrogenase: surface localization, enzymatic activity, and protein-protein interactions.
Canadian journal of microbiology. 49(5):350-6 [PMID] 12897829.
View on: PubMed
2001
Characterization of the sat operon in Streptococcus mutans: evidence for a role of Ffh in acid tolerance.
Journal of bacteriology. 183(8):2543-52 [PMID] 11274114.
View on: PubMed
2001
The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis.
Oral microbiology and immunology. 16(3):153-62 [PMID] 11358537.
View on: PubMed
2000
IVIAT: a novel method to identify microbial genes expressed specifically during human infections.
Trends in microbiology. 8(7):336-9 [PMID] 10878769.
View on: PubMed
2000
Monoclonal antibody-mediated modulation of the humoral immune response against mucosally applied Streptococcus mutans.
Infection and immunity. 68(4):1796-805 [PMID] 10722566.
View on: PubMed
1999
Streptococcus mutans ffh, a gene encoding a homologue of the 54 kDa subunit of the signal recognition particle, is involved in resistance to acid stress.
Microbiology (Reading, England). 145 ( Pt 2):357-366 [DOI] 10.1099/13500872-145-2-357. [PMID] 10075418.
1999
Virulence of a spaP mutant of Streptococcus mutans in a gnotobiotic rat model.
Infection and immunity. 67(3):1201-6 [PMID] 10024561.
View on: PubMed
1998
Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule.
Infection and immunity. 66(9):4274-82 [PMID] 9712778.
View on: PubMed
1996
Oral diseases, mycology and periodontal microbiology of HIV-1-infected women.
Oral microbiology and immunology. 11(6):371-80 [PMID] 9467369.
View on: PubMed
1993
Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans.
Infection and immunity. 61(4):1547-52 [PMID] 8454362.
View on: PubMed
1992
Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1.
Infection and immunity. 60(3):1008-17 [PMID] 1541515.
View on: PubMed
1992
Molecular, immunological and functional characterization of the major surface adhesin of Streptococcus mutans.
Advances in experimental medicine and biology. 327:229-41 [PMID] 1295342.
View on: PubMed
1991
Group B streptococcal C protein-associated antigens: association with neonatal sepsis.
The Journal of infectious diseases. 163(4):786-91 [PMID] 2010631.
View on: PubMed
1991
Identification of monoclonal antibody-binding domains within antigen P1 of Streptococcus mutans and cross-reactivity with related surface antigens of oral streptococci.
Infection and immunity. 59(12):4425-35 [PMID] 1937801.
View on: PubMed
1991
Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates.
Infection and immunity. 59(5):1803-10 [PMID] 1673448.
View on: PubMed
1990
Cloning and inactivation of the gene responsible for a major surface antigen on Streptococcus mutans.
Archives of oral biology. 35 Suppl:15S-23S [PMID] 2088219.
View on: PubMed
1990
Colloidal gold immunolabeling of immunoglobulin-binding sites and beta antigen in group B streptococci.
Infection and immunity. 58(2):332-40 [PMID] 2404866.
View on: PubMed
1989
Identification of non-immunoglobulin A-Fc-binding forms and low-molecular-weight secreted forms of the group B streptococcal beta antigen.
Infection and immunity. 57(5):1573-81 [PMID] 2651313.
View on: PubMed
1988
Two novel antigens associated with group B streptococci identified by a rapid two-stage radioimmunoassay.
The Journal of infectious diseases. 158(5):965-72 [PMID] 3053930.
View on: PubMed

Grants

Jun 2020 ACTIVE
NMR console upgrade for structural biology and metabolomics
Role: Faculty
Funding: NATL INST OF HLTH OD
Jul 2019 ACTIVE
Intracellular Invasion by Streptococcus mutans: Significance in Disease
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDCR
Mar 2017 ACTIVE
Functional amyloid formation in streptococcus mutans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jul 2016 – Jun 2021
Comprehensive Training Program in Oral Biology
Role: Other
Funding: NATL INST OF HLTH NIDCR
Jul 2016 – Jun 2021
Comprehensive Training Program in Oral Biology
Role: Other
Funding: NATL INST OF HLTH NIDCR
Apr 2016 – Mar 2019
eDNA secretion and eDMA-cell interactions in biofilm formation
Role: Principal Investigator
Funding: LOUISIANA STATE UNIV via NATL INST OF HLTH
Dec 2015 ACTIVE
MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Mar 2012 – Feb 2017
Functional Amyloid Formation in Streptococcus mutans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jul 2009 – Nov 2015
MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR

Patents

Issued September 2009
Methods and Compositions for Diagnosing and Preventing a Group B Streptococcal Infection
#7,588,773
Issued June 1998
Cloning of Non-IGA FC Binding Forms of the Group B Streptococcal Beta Antigens
#5,766,606
Issued February 1998
Gamma Antigen of Pathogenic Group B Streptococci
#5,721,339
Issued January 1997
Cloning of Non-iga Fc Binding Forms of the Group B Streptococcal Beta Antigens
#5,595,740
Issued December 1995
Novel Antigen of Group B Streptococci (CIP)
#5,472,696
Issued March 1992
Novel Bacterial Markers For Pathogenic Group B Streptococci
#5,098,827

Teaching Profile

Courses Taught
2006,2010-2012,2012-2018,2016-2021,2018-2022,2020-2022
GMS6040 Host-Pathogen Interactions
2011-2012,2014-2021,2020-2021
DEN6128 Host Defense
2011-2021
DEN5127 Infectious Diseases
2010,2013-2016,2016-2019,2018-2019
DEN8290 Special Topics
2018
GMS7980 Research for Doctoral Dissertation
2007,2009-2016
GMS6193 Research Conference in Oral Biology
2014
GMS6121 Infectious Diseases
2021
GMS6162 Oral Microbiology and Immunology

Contact Details

Phones:
Business:
(352) 273-8839
Emails: